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Correlation between IRX3 and FTO Gene Expression in Visceral and Subcutaneous Adipose Tissue in Adults Undergoing Abdominal Surgery
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RS Saba    , HR Aghazadeh , A Delbarpour , M Hedayati , M Samsam shariat , M Zarkesh |
| Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Molecular Biology, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran , zarkesh@endocrine.ac.ir |
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Abstract: (12 Views) |
Introduction: Adipose tissue is associated with energy metabolism and obesity. FTO and IRX3 genes are important genetic factors in lipid regulation and energy homeostasis. This study investigated the correlation between the expression of these genes in visceral and subcutaneous adipose tissue in Iranian adults. Materials and Methods: Fat and blood samples were collected from patients undergoing abdominal surgery, and gene expression was measured by Real-time PCR. Their relationship between their anthropometric and biochemical indices was examined by the Spearman test. Results: Of a total of 176 patients(23.9% male and 76.1% female), the relative expression of IRX3 gene in visceral adipose tissue (2.86-fold change) was significantly reduced compared to subcutaneous tissue(5.09-fold change, P<0.001), but FTO expression in subcutaneous tissue(2.35-fold change) was significantly reduced compared to visceral (3.38-fold change, P=0.039). A significant negative correlation was observed between IRX3 and FTO in subcutaneous tissue(r=-0.272, P=0.001), between visceral IRX3 and subcutaneous FTO(r=-0.344, P<0.001), and also between both genes in visceral tissue(r=-0.437, P<0.001), but there was no significant correlation between subcutaneous IRX3 and visceral FTO (r=0.065, P=0.460). The interaction between IRX3 and FTO, observed only in subcutaneous tissue, showed a significant inverse correlation with wrist circumference (r=0.196, P=0.032) and an inverse trend with triglycerides (r=0.139, P=0.097) and insulin (r=0.148, P=0.078). Conclusion: The results of the present study indicate a complex interaction and possibly mutual regulation between the IRX3 and FTO genes in visceral and subcutaneous adipose tissues. |
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| Keywords: Visceral adipose tissue, Subcutaneous adipose tissue, Gene expression, FTO gene, IRX3 gene |
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Full-Text [PDF 1165 kb]
(13 Downloads)
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Type of Study: Original |
Subject:
Genetic
Received: 2025/07/2 | Accepted: 2025/10/11 | Published: 2025/03/30
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