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Effects of Oral Sodium Nitrate Administration on Cell Blood Count in Obese Type 2 Diabetic Male Rats
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Vajiheh Khorasani   , Parichehr Yaghmeai , Maryam Tohidi , Sevda Gheibi , Tarlan Varzandi , Asghar Ghasemi |
| Research institute for Endocrine Sciences , ghasemi@endocrine.ac.ir |
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Abstract: (6291 Views) |
| Introduction: Type 2 diabetes is one of the most common metabolic diseases. Nitrate has been introduced as a new therapeutic agent for type 2 diabetes. Considering that both diabetes and nitrate have some effects on blood cell count and 30% of diabetic patients have anemia, the aim of this study was to determine the effect of sodium nitrate on blood cell count in obese type 2 diabetic rats. Materials and Methods: Forty-eight male Wistar rats were divided into four groups: Control, Control + nitrate, Diabetes and Diabetes + nitrate. The groups that received nitrate (Control + nitrate, Diabetes + nitrate) again were divided into two subgroups, which received sodium nitrate (100 and 250 mg/L in drinking water) for two months: control+nitrate100 (CN100), control+nitrate 250 (CN250), diabetes+nitrate100 (DN100), and diabetes+nitrate250 (DN250). Diabetes was induced using a high-fat diet for 14 days and injection of streptozotocin. Blood cell count was performed at the end of the study. Results: In diabetic rats, nitrate administration reduced body weight, blood glucose, hematocrits, and neutrophils (all p<0.05) but increased total number of white blood cells and lymphocytes (p<0.05). Nitrate administration had no effect on the number of red blood cells, hemoglobin concentration, MCV, MCH, MCHC, or platelet numbers. Conclusion: Administration of sodium nitrate, which is considered as a therapeutic agent in type 2 diabetes, decreased blood glucose in the type 2 diabetic rats but had no major harmful effects on blood parameters; in addition, it may also have anti-inflammatory effects by decreasing the number of neutrophils. |
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| Keywords: Type 2 diabetes, Nitrate, Cell blood count, Male rat |
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Full-Text [PDF 466 kb]
(1316 Downloads)
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Type of Study: Original |
Subject:
Physiology
Received: 2017/08/8 | Accepted: 2017/10/21 | Published: 2018/01/21
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