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:: Volume 14, Issue 5 (1-2013) ::
2013, 14(5): 484-491 Back to browse issues page
The Anti-Obesity Effect of D-Lys3- GHRP-6 Peptide, GHSR Receptor Antagonist in Rats
Homayoun Khazali Dr , Fariba Mahmoudi
shahid beheshti university , faribamahmoudi_14@yahoo.com
Abstract:   (8522 Views)
Introduction: Neuropeptide Y (NPY) neurons express growth hormone secretagogues receptor (GHSR-Ia). Fasting or ghrelin increases food intake by stimulating NPY gene expression via GHSR-Ia. D-Lys3 -GHRP-6, a ghrelin receptor antagonist, decreases food intakes and body weight. The present study aimed to investigate the molecular mechanism of the anorexigenic effect of D-Lys3 -GHRP-6 in food deprived rats. Materials and Methods: Fifteen food deprived rats (3 groups) received central injections of saline or D-Lys3 -GHRP-6 (2 or 20nmol) respectively and mean food intake was measured at one hour after injections. Mean plasma ghrelin concentration was measured by RIA, and NPY gene expression was determined by semi quantitative RT-PCR in the hypothalamus. Fifteen rats in three groups, received central injection of saline or initiations and on the D-Lys3 -GHRP-6 (2 or 20nmol) twice daily for 9 days respectively. Body weight was determined at third, 7th and 10th day of the experiment. Results: Mean plasma ghrelin concentration, mean food intake and NPY gene expression significantly increased in food deprived rats compared to fed animals. D-Lys3 –GHRP-6 (2 nmol) did not significantly change ghrelin, food intake and NPY mRNA levels, compared to the food deprived rats. However D-Lys3 –GHRP-6 (20 nmol) significantly decreased ghrelin, food intake and NPY mRNA level compared to food deprived group. Conclusion: Results showed that D-Lys3 -GHRP-6 may exert its anorexigenic effect on food intake by influencing the expression of peptides involved in the regulation of energy homeostasis, and may be suggested for the treatment of obesity.
Keywords: Ghrelin, NPY, D-Lys3 -GHRP-6, Food deprivation
Full-Text [PDF 324 kb]   (1917 Downloads)    
Type of Study: Original | Subject: Endocrinology
Received: 2012/03/27 | Accepted: 2012/07/2 | Published: 2014/05/17
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Khazali H, Mahmoudi F. The Anti-Obesity Effect of D-Lys3- GHRP-6 Peptide, GHSR Receptor Antagonist in Rats. Iranian Journal of Endocrinology and Metabolism. 2013; 14 (5) :484-491
URL: http://ijem.sbmu.ac.ir/article-1-1362-en.html


Volume 14, Issue 5 (1-2013) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
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