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The Anti-Obesity Effect of D-Lys3- GHRP-6 Peptide, GHSR Receptor Antagonist in Rats
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Homayoun Khazali   , Fariba Mahmoudi |
| shahid beheshti university , faribamahmoudi_14@yahoo.com |
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Abstract: (11224 Views) |
| Introduction: Neuropeptide Y (NPY) neurons express growth hormone secretagogues receptor (GHSR-Ia). Fasting or ghrelin increases food intake by stimulating NPY gene expression via GHSR-Ia. D-Lys3 -GHRP-6, a ghrelin receptor antagonist, decreases food intakes and body weight. The present study aimed to investigate the molecular mechanism of the anorexigenic effect of D-Lys3 -GHRP-6 in food deprived rats. Materials and Methods: Fifteen food deprived rats (3 groups) received central injections of saline or D-Lys3 -GHRP-6 (2 or 20nmol) respectively and mean food intake was measured at one hour after injections. Mean plasma ghrelin concentration was measured by RIA, and NPY gene expression was determined by semi quantitative RT-PCR in the hypothalamus. Fifteen rats in three groups, received central injection of saline or initiations and on the D-Lys3 -GHRP-6 (2 or 20nmol) twice daily for 9 days respectively. Body weight was determined at third, 7th and 10th day of the experiment. Results: Mean plasma ghrelin concentration, mean food intake and NPY gene expression significantly increased in food deprived rats compared to fed animals. D-Lys3 –GHRP-6 (2 nmol) did not significantly change ghrelin, food intake and NPY mRNA levels, compared to the food deprived rats. However D-Lys3 –GHRP-6 (20 nmol) significantly decreased ghrelin, food intake and NPY mRNA level compared to food deprived group. Conclusion: Results showed that D-Lys3 -GHRP-6 may exert its anorexigenic effect on food intake by influencing the expression of peptides involved in the regulation of energy homeostasis, and may be suggested for the treatment of obesity. |
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| Keywords: Ghrelin, NPY, D-Lys3 -GHRP-6, Food deprivation |
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Full-Text [PDF 324 kb]
(2952 Downloads)
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Type of Study: Original |
Subject:
Endocrinology
Received: 2012/03/27 | Accepted: 2012/07/2 | Published: 2014/05/17
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