:: Volume 19, Issue 5 (1-2018) ::
2018, 19(5): 340-348 Back to browse issues page
Effects of Oral Sodium Nitrate Administration on Cell Blood Count in Obese Type 2 Diabetic Male Rats
Vajiheh Khorasani, Parichehr Yaghmeai, Maryam Tohidi, Sevda Gheibi, Tarlan Varzandi, Asghar Ghasemi
Research institute for Endocrine Sciences , ghasemi@endocrine.ac.ir
Abstract:   (5676 Views)
Introduction: Type 2 diabetes is one of the most common metabolic diseases. Nitrate has been introduced as a new therapeutic agent for type 2 diabetes. Considering that both diabetes and nitrate have some effects on blood cell count and 30% of diabetic patients have anemia, the aim of this study was to determine the effect of sodium nitrate on blood cell count in obese type 2 diabetic rats. Materials and Methods: Forty-eight male Wistar rats were divided into four groups: Control, Control + nitrate, Diabetes and Diabetes + nitrate. The groups that received nitrate (Control + nitrate, Diabetes + nitrate) again were divided into two subgroups, which received sodium nitrate (100 and 250 mg/L in drinking water) for two months: control+nitrate100 (CN100), control+nitrate 250 (CN250), diabetes+nitrate100 (DN100), and diabetes+nitrate250 (DN250). Diabetes was induced using a high-fat diet for 14 days and injection of streptozotocin. Blood cell count was performed at the end of the study. Results: In diabetic rats, nitrate administration reduced body weight, blood glucose, hematocrits, and neutrophils (all p<0.05) but increased total number of white blood cells and lymphocytes (p<0.05). Nitrate administration had no effect on the number of red blood cells, hemoglobin concentration, MCV, MCH, MCHC, or platelet numbers. Conclusion: Administration of sodium nitrate, which is considered as a therapeutic agent in type 2 diabetes, decreased blood glucose in the type 2 diabetic rats but had no major harmful effects on blood parameters; in addition, it may also have anti-inflammatory effects by decreasing the number of neutrophils.
Keywords: Type 2 diabetes, Nitrate, Cell blood count, Male rat
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Type of Study: Original | Subject: Physiology
Received: 2017/08/8 | Accepted: 2017/10/21 | Published: 2018/01/21

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