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Investigating the Effects of Rosiglitazone and Epigallocatechin-3-Gallate on PGC-1α protein expression in Palmitate-induced Insulin Resistant C2C12 skeletal Muscle Cells
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Salar Bakhtiyari   , Azar Babakhani , Karimeh Haghani |
| Ilam University of Medical Sciences , sobhe14@yahoo.com |
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Abstract: (9061 Views) |
Introduction: Alterations in lipids
metabolism are one of the important mechanisms for the treatment of insulin
resistance and, hence for type 2 diabetes. On the other hand, PGC-1 supportFields]> QUOTE
12?±">
 α supportFields]>, as a key regulator of
mitochondrial biogenesis and function, by increasing β-oxidation of lipids
plays an important role in improving insulin sensitivity. In the present study,
the effects of Epigallocatechin-3-Gallate (EGCG), an anti-obesity and enhancer
of lipid catabolism agent, on PGC-1 supportFields]> QUOTE
12?±">
α supportFields]> protein expression was
examined and compared with the anti-diabetic drug Rosiglitazone (RGZ). Materials
and Methods: After differentiation of C2C12 myoblasts to myotubes, insulin
resistance was induced by palmitate treatment. Afterward, PGC-1α protein expression
was examined using the western blot method before and after treatment with
insulin and after EGCG and RGZ treatment. Results: Palmitate treatment
significantly decreased PGC-1 supportFields]> QUOTE
12?±">
α supportFields]>protein expression in the
C2C12 cells (P=0.001). Treatment of these cells with EGCG had no significant
effect on the PGC-1 supportFields]>
QUOTE
12?±">
α supportFields]> protein expression (P=0.67),
whereas treatment with RGZ significantly increased expression of this gene at
protein level (P=0.003). In addition, this significant increase in PGC-1 supportFields]> QUOTE
12?±">
α supportFields]> protein expression was maintained
by simultaneous treatment with EGCG and RGZ (P=0.001). Conclusion:
Our results showed that the effect of EGCG on PGC-1 supportFields]> QUOTE
12?±">
α supportFields]> protein expression was not
significant, whereas RGZ significantly improved the palmitate-induced reduction
of PGC-1 supportFields]>
QUOTE
12?±">
α supportFields]> protein expression. Overall,
it seems that anti-diabetic effect of EGCG is not exerted through its effect on
the expression of PGC-1 supportFields]>
QUOTE
12?±">
α supportFields]> gene, in contrast to that of RGZ. |
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| Keywords: Insulin resistance, C2C12 muscle cells, PGC-1α gene, Rosiglitazone, Epigallocatechin-3-Gallate |
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Full-Text [PDF 600 kb]
(2533 Downloads)
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Type of Study: Original |
Subject:
Biochemistry
Received: 2014/09/9 | Accepted: 2015/02/4 | Published: 2015/05/26
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