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:: Volume 17, Number 1 (6-2015) ::
2015, 17(1): 53-62 Back to browse issues page
Investigating the Effects of Rosiglitazone and Epigallocatechin-3-Gallate on PGC-1α protein expression in Palmitate-induced Insulin Resistant C2C12 skeletal Muscle Cells
Dr. Salar Bakhtiyari, Azar Babakhani, Dr. Karimeh Haghani *
Assistant professor Ilam University of Medical Sciences , sobhe14@yahoo.com
Abstract:   (2891 Views)

Introduction: Alterations in lipids metabolism are one of the important mechanisms for the treatment of insulin resistance and, hence for type 2 diabetes. On the other hand, PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]>, as a key regulator of mitochondrial biogenesis and function, by increasing β-oxidation of lipids plays an important role in improving insulin sensitivity. In the present study, the effects of Epigallocatechin-3-Gallate (EGCG), an anti-obesity and enhancer of lipid catabolism agent, on PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]> protein expression was examined and compared with the anti-diabetic drug Rosiglitazone (RGZ). Materials and Methods: After differentiation of C2C12 myoblasts to myotubes, insulin resistance was induced by palmitate treatment. Afterward, PGC-1α protein expression was examined using the western blot method before and after treatment with insulin and after EGCG and RGZ treatment. Results: Palmitate treatment significantly decreased PGC-1 supportFields]> QUOTE 12"> AWT IMAGE  α supportFields]>protein expression in the C2C12 cells (P=0.001). Treatment of these cells with EGCG had no significant effect on the PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]> protein expression (P=0.67), whereas treatment with RGZ significantly increased expression of this gene at protein level (P=0.003). In addition, this significant increase in PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]> protein expression was maintained by simultaneous treatment with EGCG and RGZ (P=0.001). Conclusion: Our results showed that the effect of EGCG on PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]> protein expression was not significant, whereas RGZ significantly improved the palmitate-induced reduction of PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]> protein expression. Overall, it seems that anti-diabetic effect of EGCG is not exerted through its effect on the expression of PGC-1 supportFields]> QUOTE 12"> AWT IMAGE α supportFields]> gene, in contrast to that of RGZ.     

Keywords: Insulin resistance, C2C12 muscle cells, PGC-1α gene, Rosiglitazone, Epigallocatechin-3-Gallate
Full-Text [PDF 600 kb]   (713 Downloads)    
Type of Study: Original | Subject: Biochemistry
Received: 2014/09/9 | Accepted: 2015/02/4 | Published: 2015/05/26
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Bakhtiyari S, Babakhani A, Haghani K. Investigating the Effects of Rosiglitazone and Epigallocatechin-3-Gallate on PGC-1α protein expression in Palmitate-induced Insulin Resistant C2C12 skeletal Muscle Cells . Iranian Journal of Endocrinology and Metabolism. 2015; 17 (1) :53-62
URL: http://ijem.sbmu.ac.ir/article-1-1794-en.html
Volume 17, Number 1 (6-2015) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
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