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The Effect of Aerobic Exercise and Probiotic Supplementation on HDAC6 Gene Expression in Adipose Tissue of Obese Male Rats Induced by a High-Fat and Fructose Diet
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M Karimi BabaAhmadi    , A Kaki , E Larki Ghorbani |
| Department of Sport Physiology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran, , ahvaz.kaki@yahoo.com |
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Abstract: (70 Views) |
Introduction: Obesity is one of the most significant public health challenges worldwide and is associated with metabolic disorders, chronic inflammation, and epigenetic changes, including increased expression of the HDAC6 gene. HDAC6, as a key epigenetic regulator, plays a prominent role in adipose tissue by contributing to insulin resistance and mitochondrial dysfunction through its involvement in inflammatory and cellular stress pathways. This study aimed to investigate the effects of aerobic exercise and probiotic supplementation on HDAC6 gene expression in the visceral adipose tissue of male Wistar rats with diet-induced obesity. Materials and Methods: In this experimental study, 40 eight-week-old male Wistar rats were randomly assigned to either a standard diet or a high-fat, high-fructose (HFHF) diet. After 24 weeks of obesity induction, the animals were divided into five subgroups: control, obese, obese + exercise, obese + probiotic, and obese + exercise + probiotic. Aerobic exercise was performed on a treadmill for 8 weeks, and probiotics were administered orally. At the end of the intervention, HDAC6 gene expression in visceral adipose tissue was measured using Real-Time PCR. Results: The HFHF diet significantly increased HDAC6 gene expression and metabolic indices compared to the control group (P=0.001). In contrast, aerobic exercise, probiotic supplementation, and especially their combination significantly reduced these parameters (P=0.001). Conclusion: These findings indicate that aerobic exercise and probiotics, particularly in combination, may effectively modulate epigenetic pathways and reduce the metabolic complications associated with diet-induced obesity. |
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| Keywords: Aerobic exercise, Probiotic, HDAC6 gene, Adipose tissue, Obesity |
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Full-Text [PDF 1017 kb]
(64 Downloads)
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Type of Study: Original |
Subject:
Exercise
Received: 2025/07/19 | Accepted: 2026/02/1 | Published: 2025/03/30
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