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The Protective Effect of Eight Weeks of Caffeine Supplementation on Changes in Apoptotic Factors Induced by High-fat Diet in Rats
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B Amiri    , MR Rahimi , Sh Ahmadi , H Faraji |
| Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran. , r.rahimi@uok.ac.ir |
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Abstract: (137 Views) |
Introduction: A high-fat diet (HFD) contributes to obesity and cardiac damage. This study investigated the protective effects of eight weeks of caffeine supplementation (CAF) on HFD-induced changes in the expression of BAX, BCL2, the BAX/BCL2 ratio, and caspase 3 in the heart tissue of male Wistar rats. Materials and Methods: Thirty-two male Wistar rats (8 weeks old, 181 ± 15 grams) were randomly assigned to one of four groups (n=8 per group): normal diet (ND), normal diet with caffeine (ND+CAF), high-fat diet (HFD), and high-fat diet with caffeine (HFD+CAF). Rats had free access to water and their assigned diet. The caffeine supplement was administered via gavage at 6 mg/kg/day. After eight weeks, heart tissue was collected, and the protein expression of apoptosis markers was measured using Western blotting. Data were analyzed using one-way ANOVA followed by Tukey’s post-hoc test (GraphPad Prism). Results: Compared to the ND and ND+CAF groups, the HFD and HFD+CAF groups showed significantly higher BAX expression (P≤0.001). BCL2 expression was significantly elevated in the ND+CAF and HFD+CAF groups compared to the ND and HFD groups (P≤0.001). The BAX/BCL2 ratio was lower in the ND+CAF and HFD+CAF groups compared to the ND and HFD groups (P ≤ 0.001). No significant differences were observed in caspase 3 expression (P≥0.05). Conclusion: These findings suggest that caffeine supplementation may mitigate HFD-induced alterations in the expression of specific apoptotic proteins in rat heart tissue |
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| Keywords: Caffeine, High-fat diet, BAX, BCL2, Caspase 3 |
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Full-Text [PDF 1157 kb]
(102 Downloads)
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Type of Study: Original |
Subject:
Nutrition
Received: 2024/07/7 | Accepted: 2024/11/18 | Published: 2024/04/29
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