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The effect of Vitamin E effect on diabetes mellitus induced changes in the small intestine of rats
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AR Shirpoor   , B Ilkhanizadeh , S Saadatian , M Eskandari , F Ghaderi Pakdel , M Karimipoor , A Gol , A Poor Shahbazi |
| , shirpoor@hotmail.com |
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Abstract: (27799 Views) |
| Introduction: Diabetes Mellitus (DM), as a metabolic disease, causes numerous effects on the vascular, ocular, renal, neuronal, gastrointestinal and other systems. In this study the effects of vitamin E (vit. E) on DM induced changes in the small intestine of rats were studied morphologically and histologically. Materials and Methods: 24 wistar male rats (average weight 250gr, average age 8 months) were randomly selected. Diabetes Mellitus was induced by STZ (60 mg/Kg, i. p.) in 16 rats. All animals were classified in to 3 groups (n=8): control or non diabetic rats, diabetic non-treated, and diabetic treated by vit. E. In treated rats, vit. E (300 mg) was added to tap water. Animals were sacrificed following deep anesthesia using Hydrate chloral (0. 5 mL/100 gr B. W, i. p) at the end of 6th week of experimental protocols. The small intestines of all rats were cut after the mesenteric layers were precisely detached from the pyloric and illeocecal sphincters. Weight and length of intestines were assessed, and then 5 cm long samples were cut from the duodenum, jejunum and the ileum. Each sample was filled with 10% formalin buffer and after ligature of their two ends they were kept in 10% formalin buffer. The samples were histologically studied for villi length, crypt depth and muscular thickness. Results: The results showed that intestinal length and weight, villi length, crypts depth and muscular thickness were increased in comparison with the control, non diabetic rats significantly. In the rats treated with vit.E, there were no differences in the mentioned items, in comparison with the control group. Conclusion: The results of this study demonstrate that vit. E as an antioxidant with decreasing effects on free radicals and oxidative stress, attenuates or prevents certain diabetic disorders. |
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| Keywords: Diabetes, Small intestine, Vit E, Rat |
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Full-Text [PDF 335 kb]
(2906 Downloads)
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Type of Study: Original |
Subject:
Endocrinology
Received: 2006/11/30 | Published: 2004/12/15
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