Introduction: The glucagon-like peptide-1 (GLP-1), is a neuropeptide and an incretin that helps regulate glucose and insulin concentrations. The effects of acute aerobic exercise however on the response of GLP-1 are unclear. The purpose of this study was to investigate the effect of acute aerobic exercise on glucagon -like peptide-1 (GLP-1) serum levels, glucose and insulin in women with type II diabetes mellitus. Materials and Methods: In this study, 20 patients with type II diabetes were enrolled in two groups (experimental and control). The experimental group underwent aerobic training one times (running on treadmill) with moderate intensity 65-75% HR max to exhaustion. Blood samples before exercise, immediately after and 24 hours after exercise were collected for measuring GLP-1, glucose and Insulin. Results: Results showed that none of sampling time effects on GLP-1, glucose and insulin were significant (p>0.05). also, Pearson correlation showed that was significant relationship between GLP-1 changes levels and glucose (p<0.05). Conclusion: Results indicate that in women with type II diabetes, acute aerobic exercise, with moderate intensity to exhaustion immediately and 24 hours after exercise has no effect on serum levels of GLP-1, glucose and insulin.