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:: Volume 18, Number 3 (9-2016) ::
2016, 18(3): 187-196 Back to browse issues page
The Study of cell Proliferation, TNF-α Cytokine Production and Expression of CD8 Marker in Peripheral Blood Lymphocytes of Women with Polycystic Ovary Syndrome (PCOS) by co-culture with Ovarian Tumor Cell Lines (SKOV3,A2780)
Mehri Hajiaghayi, Fatemeh Rezayat, Nazanin Ghasemi, Amin Soleymanian, Mehrnaz Mesdaghi, Dr Nariman Mosaffa *, Dr Fahimeh Ramezani Tehrani
PhD Shahid Beheshti University of Medical Sciences, Tehran, Iran. , yasamaryan@gmail.com
Abstract:   (2123 Views)

Introduction: Polycystic ovarian syndrome (PCOS) is a proinflammatory state that underpins the development of metabolic aberration and ovarian dysfunction in the disorder. Chronic inflammation and increased levels of androgens in these patients and their impact on the immune system, may be able to disrupt antitumor activity and thus increase the risk of developing malignancies including ovarian cancer. Materials and Methods: Peripheral blood mononuclear cells of 50 patients with PCOS and healthy controls were purified by Ficoll density gradient centrifugation. We then measured cell proliferation and concentrations of cytokines TNF-α at different time intervals (48 and 72 hours) after co-culture of ovarian (SKOV3, A2780) and breast (MCF-7, MDA-468) tumor cell lines with PBMC in indirect contact of transwell system. Results: Proliferative response of executive cells during stimulation with tumor cell lines after 48 hours was not statistically significant between patients and healthy controls. Between the 2 groups,  proliferation rates at the end of 72h were significantly higher than after 48h (P<0.01). The production of TNF-α in co-culture of A2780 cell lines significantly increased in the patient group in time compared to the controls (P<0.05). Conclusion: Our findings confirmed that, compared to healthy individuals low levels of chronic inflammation in patients with PCOS exhibit increased proliferative response of immune cells and TNF-α levels. An increased risk of cancers in patients with PCOS however requires investigation of other aspects of anti-tumor responses in vitro, with larger sample sizes.

Keywords: Polycystic ovarian syndrome, Chronic inflammation, Ovarian tumor cell lines, SKOV3, A2780, Co-culture
Full-Text [PDF 529 kb]   (425 Downloads)    
Type of Study: Original | Subject: Endocrinology
Received: 2016/04/11 | Accepted: 2016/06/1 | Published: 2016/09/6
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Hajiaghayi M, Rezayat F, Ghasemi N, Soleymanian A, Mesdaghi M, Mosaffa N et al . The Study of cell Proliferation, TNF-α Cytokine Production and Expression of CD8 Marker in Peripheral Blood Lymphocytes of Women with Polycystic Ovary Syndrome (PCOS) by co-culture with Ovarian Tumor Cell Lines (SKOV3,A2780). Iranian Journal of Endocrinology and Metabolism. 2016; 18 (3) :187-196
URL: http://ijem.sbmu.ac.ir/article-1-2080-en.html
Volume 18, Number 3 (9-2016) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
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