Introduction: Diabetes and its complications have a significant impact on patients' quality of life and spirituality can help improve the quality of life in such patients. The purpose of this study was to determine the relationship of spirituality with quality of life in patients with Type 2 diabetes. Materials and Methods: This was a descriptive-correlation study. Participants were 145 individuals with type 2 diabetes, patients of rural health clinics and the Valiasr hospital in Khorramshahr city, selected via stratified random and sampling methods. Data collection tools were a version of 29-item of the spiritual questionnaire (Parsian and Dunning) and a version of 26-item of the Quality of Life Questionnaire (World Health Organization), both questionnaires with previously confirmed validity and reliability in numerous studies. Data were analyzed using SPSS version 16 software and Pearson's correlation, T-test, ANOVA, Post Hoc and Stepwise Regression analytical statistics. Results: There was a significant positive relationship between spirituality and quality of life (p<0.0001). The results show a statistically significant relationship between spirituality and quality of life with both education and age. Conclusion: A strong correlation between spirituality and quality of life in patients with type II diabetes, demonstrates the roles and responsibilities of healthcare providers, including physicians, nurses and patients' families in meeting the varied spiritual and religious needs of patients along with their therapeutic management. Focusing on improving spiritual health is also important in education programs for these patients.