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:: Editorial Board
:: About Iranian Journal of Endocrinology and Metabolism
:: Volume 15, Issue 3 (10-2013) ::
2013, 15(3): 311-321 Back to browse issues page
The Effect of Obesity and Endurance Training-induced Weight Loss on UCP3 mRNA Expression in C57BL/6 MICE
Hamid Mohebbi , Hadi Rohani , Sadegh Hassan-nia , Nazanin Pirooznia
University of Guilan , mohebbi_h@yahoo.com
Abstract:   (10047 Views)


Introduction: The purpose of this study was evaluating the effect of obesity and weight reduction induced by endurance training on UCP3 mRNA expression in C57BL/6 mice. Materials and Methods: Twenty-four C57BL/6 mice consumed a high-fat diet, while 6 (NB Group) were fed a normal chow for 12 weeks. After the fattening period, 6 obese mice (OB Group) were sacrificed. Twelve mice trained 5 sessions per week for 12 weeks on a rodent treadmill with high (HI Group) (~75% VO2max) and/or low intensity (LI Group) (~50% VO2max) during a training period. The control group (OC Group) was kept inactive in the cages till the end of the period. Body weight was measured and recorded weekly. UCP3 mRNA expression was measured in gastrocnemius muscle using Real Time-PCR method and plasma FFA concentrations were measured using the ELISA method. Results: In the training groups, weight reduction was significant in the 8th week (p<0.05). Average relative UCP3 mRNA expression was significantly lower in the HI and LI groups, than in the OC group (0.5±0.25 and 1.04±0.96, respectively, vs. 2.58±0.57 arbitrary unit p<0.05). Plasma FFA conce­ntrations were significantly lower in the HI and OB groups compared to the LI group (p<0.05). Conclusion: When energy intake and consequently body weight increases, UCP3 increases to extend the thermogenesis and fatty acid oxidation’s process. In contrast, UCP3 downregulates with exercise-induced weight loss in order to counteract this process.

Keywords: Obesity, Weight loss, Endurance training, UCP3 mRNA
Full-Text [PDF 380 kb]   (3627 Downloads)    
Type of Study: Original | Subject: Exercise
Received: 2013/01/21 | Accepted: 2013/09/28 | Published: 2013/09/28
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Mohebbi H, Rohani H, Hassan-nia S, Pirooznia N. The Effect of Obesity and Endurance Training-induced Weight Loss on UCP3 mRNA Expression in C57BL/6 MICE. Iranian Journal of Endocrinology and Metabolism 2013; 15 (3) :311-321
URL: http://ijem.sbmu.ac.ir/article-1-1502-en.html

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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 15, Issue 3 (10-2013) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
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