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The Effect of Fetal Hypothyroidism on the Serum and Tissue Levels of Nitric Oxide Metabolites in Adult Male Hypothyroid Progeny
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Fatemeh Mehrazin   , Abdolhossein Shiravi , Saleh Zahediasl , Asghar Ghasemi |
| مرکز تحقیقات غدد، پژوهشکده علوم غدد درون ریز و متابولیسم، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران , Ghasemi@endocrine.ac.ir |
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Abstract: (11652 Views) |
| Introduction: Decreased Nitric oxide (NO) synthesis has been reported in hypothyroidism. Nitrate consumption is associated with increased serum NO metabolites, nitrate and nitrite (NOx). The aim of this study was to evaluate NOx levels in serum and heart, aorta, and lung tissues of the offspring of hypothyroid rats. Materials and Methods: Hypothyroidism was induced in female rats using 6-n-propylthiouracil in drinking water from the beginning to the end of gestation. Fetal male offspring with and without fetal hypothyroidism were followed until adulthood and then divided into four groups including the control and hypothyroidism groups with injection of sodium nitrate (10 mg/kg, ip), and the control and hypothyroidism groups without injection. NOx concentrations in serum and tissue samples were measured using the Griess assay. Results: Serum NOx concentrations in both groups reached a maximum one hour after nitrate injection. NOx level decreased in both groups after the first hour with a steeper slope in fetal hypothyroid rats. Heart and aorta NOx concentrations were significantly lower than controls in fetal hypothyroid group. Following nitrate injection, the NOx concentration in the controls group increased in the heart and lung tissues, but significant increases were observed in all three tissues in the fetal hypothyroid group. Conclusion: NOx metabolism differs in fetal hypothyroid and control rats and the role of thyroid hormones in NOx metabolism persists until adulthood. |
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| Keywords: Fetal hypothyroidism, Nitric oxide, Rat |
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Full-Text [PDF 297 kb]
(2532 Downloads)
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Type of Study: Original |
Subject:
Endocrinology
Received: 2012/06/12 | Accepted: 2013/03/6 | Published: 2013/03/6
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