Introduction: Esophageal cancer is one of the most common cancers in Iran and has a high mortality rate. The aim of this study was to assess the association between folate and vitamin B12 intake and risk of squamous-cell carcinoma of the esophagus cancer. Materials and Methods: In this case-control study, cases (n=47) were patients diagnosed with squamous-cell carcinoma of the esophagus (SCCE) in main hospital Kurdistan and controls (matched with cases by age and sex) were patients with acute non-cancer diseases at the same hospitals (n=96). Demographic data and physical activity were collected using questionnaires. A semi-quantitative food frequency questionnaire used for nutritional information and folate and vitamin B12 intake were calculated with Nutritionst IV (N4). Results: Thirty eight percent of cases and 40% of controls were males. Mean age of cases and controls were 58±18 and 58±17 years respectively. Mean folate intake among cases was 152.1 μg/day, which was less than in the controls. After adjustment for confounders, folate intake was inversely related with SCCE (OR=0.03, 95% CI 0.00, 0.12). The association between vitamin B₁₂ intake and SCCE was not significant (p=0.46). Conclusion: Folate intake was inversely related with SCCE: however, there was not a significant association between vitamin B₁₂ intake and SCCE.