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Effect of Long-term Nitrate Administration on the mRNA Expression of Sialin (SLC17A5) Gene in the Tissues of Healthy Male Rats
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F Ghorbani    , M Zarkesh , S Jeddi , A Ghasemi |
| Endocrine Physiology Research Center, Research Institute for Endocrine Molecular Biology, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran, , sajad.jeddi@sbmu.ac.ir |
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Abstract: (199 Views) |
Introduction: Nitrate requires a transporter to cross the membrane, and sialin, a nitrate transporter, plays a role in physiological processes such as entero-salivary nitrate circulation; its dysfunction is associated with diseases such as type 2 diabetes. The objective of this study is to investigate the effect of long-term administration of nitrate on the expression of sialin mRNA in the tissues (skeletal muscle, kidney, stomach, adipose tissue, aorta, liver, lung, pancreas, heart, brain, adrenal glands, and intestine) of healthy male rats. Materials and Methods: Rats were divided into two groups of 6 (control and nitrate-treated), which received drinking water and drinking water containing nitrate (100 mg/L) for 6 months, respectively. At the end of the study, sialin mRNA levels and nitrate and nitrite were measured in the tissues. Results: Nitrate decreased sialin mRNA expression in skeletal muscle (40%, P=0.032), kidney (61%, P<0.001), stomach (46%, P=0.004), and adipose tissue (57%, P=0.019). Nitrate administration increased nitrate in skeletal muscle (58%, P=0.049), adipose tissue (85%, P=0.002), aorta (44%, P=0.084), and liver (36%, P=0.085), and nitrite in skeletal muscle (76%, P=0.079), kidney (79%, P<0.001), stomach (68%, P=0.098), lung (95%, P=0.025), pancreas (93%, P=0.012), and heart (120%, P=0.007). Conclusion: The decreased sialin mRNA expression following nitrate administration in some tissues (skeletal muscle, kidney, stomach, and adipose tissue) is a form of negative feedback; however, this effect was not observed in some tissues (liver and aorta). Nitrate may regulate sialin gene expression in specific tissues. |
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| Keywords: Nitric oxide, Nitrate, Gene expression, Sialin, Rat |
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Full-Text [PDF 1005 kb]
(122 Downloads)
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Type of Study: Original |
Subject:
Physiology
Received: 2025/09/7 | Accepted: 2025/11/2 | Published: 2025/03/30
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Ghorbani F, Zarkesh M, Jeddi S, Ghasemi A. Effect of Long-term Nitrate Administration on the mRNA Expression of Sialin (SLC17A5) Gene in the Tissues of Healthy Male Rats. Iranian Journal of Endocrinology and Metabolism 2025; 27 (1) :1-11 URL: http://ijem.sbmu.ac.ir/article-1-3270-en.html
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