|
|
|
|
|
|
 |
Volume 25, Issue 5 (12-2023) |
 |
|
|
|
|
Effect of Hyperoxia on Nitric Oxide Bioavailability in Epididymal Adipose Tissue of Male Type 2 Diabetic Rats
|
A Ghasemi    , S Jeddi , R Norouzirad |
| Department of Biochemistry, School of Medicine, Dezful University of Medical Sciences, Dezful, I.R. Iran. , norouzirad@gmail.com |
|
|
Abstract: (178 Views) |
Introduction: Hypoxia, particularly in adipose tissue, is involved in the pathophysiology of type 2 diabetes. Hyperoxia, oxygen administration, has been proposed as a potential treatment for this condition. However, one complication associated with hyperoxia is the decreased bioavailability of nitric oxide (NO). This study investigates the effect of hyperoxia on NO-producing enzymes (NO synthase, NOS) and the L-arginine-degrading enzyme (arginase) in epididymal adipose tissue of type 2 diabetic rats. Material and Methods: Type 2 diabetes was induced in 12 male rats using a high-fat diet combined with a low dose of streptozotocin. The rats were then divided into two groups: hyperoxia (received 95% oxygen for five weeks) and control group exposed to air containing 21% oxygen). After five weeks, the animals were anesthetized, and epididymal adipose tissue was isolated. Protein levels of endothelial NOS (eNOS), inducible NOS (iNOS), and arginase, as well as concentrations of nitric oxide metabolites (NOx) and lactate, were measured. Results: Hyperoxia resulted in a 28% reduction in fasting serum glucose, decreased lactate concentration, decreased eNOS protein levels (11.1±5.2 vs. 8.4±1.3 ng/mg protein, P=0.041), decreased NOx levels (18.2±1.0 vs. 12.1±1.2 nmol/mg protein, P=0.027), and increased arginase protein levels (1.3±0.14 vs. 2.2±0.31 ng/ mg protein, P=0.028) in adipose tissue. Conclusion: Hyperoxia reduced fasting serum glucose in type 2 diabetic rats but simultaneously decreased NO bioavailability in visceral adipose tissue, which associated with reduced eNOS protein and increased arginase protein levels.
|
|
| Keywords: Type 2 diabetes, Nitric oxide, Visceral fat, Arginase, Hyperoxia, Rat |
|
|
Full-Text [PDF 711 kb]
(173 Downloads)
|
Type of Study: Original |
Subject:
Biochemistry
Received: 2024/07/30 | Accepted: 2024/09/16 | Published: 2023/12/31
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
|
Ghasemi A, Jeddi S, Norouzirad R. Effect of Hyperoxia on Nitric Oxide Bioavailability in Epididymal Adipose Tissue of Male Type 2 Diabetic Rats. Iranian Journal of Endocrinology and Metabolism 2023; 25 (5) :391-398 URL: http://ijem.sbmu.ac.ir/article-1-3188-en.html
|
|
|
|
|
|
|
|
|
|
|
|
|
|
