[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 23, Issue 2 (7-2021) ::
2021, 23(2): 92-101 Back to browse issues page
Expression of Luteinizing Hormone (LH) Receptor Gene in the Ovary of a Prenatally-Androgenized Rat Model of Polycystic Ovary Syndrome Following Androgen Exposure in the Prenatal Period
Mahsa Noroozzadeh , Marziyeh Salehi Jahromi , Hanieh Gholami , Fahimeh Ramezani Tehrani
Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran, , ramezani@endocrine.ac.ir
Abstract:   (204 Views)
Introduction: Based on available evidence, exposure of the female fetus to androgens during the prenatal period, by affecting the expression of some genes, can cause polycystic ovary syndrome (PCOS) in adulthood. On the other hand, changes in luteinizing hormone (LH) levels and its receptor are associated with the risk of reproductive disorders, including PCOS. The present study aimed to evaluate the expression of the LH receptor gene in the ovaries of a rat model of PCOS induced by androgen exposure in the prenatal period. Materials and Methods: In adult rats with PCOS and controls (eight animals in each group), different phases of the sexual cycle (estrous cycle) were determined using vaginal smears, and the anogenital distance (AGD) was measured using a vernier caliper. Ovarian granulosa cells were isolated, and RNA was extracted. The LH receptor gene expression levels in these cells were determined using the real-time polymerase chain reaction (PCR). Data were analyzed by GraphPad Prism software. Results: Irregular and longer sexual cycles, an increase in AGD (17.32 17 0.3 vs. 11.73 ± 0.26 mm (P=0.0001)), and a decrease in LH receptor gene expression (42. ± 0.12) 0 vs. 0.88 (0.13 (P=0.02)) were observed in the adult rat model of PCOS compared to controls. Conclusion: A decrease in LH receptor gene expression in the ovarian granulosa cells may be one of the mechanisms involved in the pathophysiology of PCOS following prenatal exposure of the female fetus to androgens.
Keywords: Androgen, Fetal life, LH receptor, Polycystic ovary syndrome, Rat
Full-Text [PDF 1053 kb]   (83 Downloads)    
Type of Study: Original | Subject: Gynecology
Received: 2021/08/12 | Accepted: 2021/12/1 | Published: 2021/07/1
Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Noroozzadeh M, Salehi Jahromi M, Gholami H, Ramezani Tehrani F. Expression of Luteinizing Hormone (LH) Receptor Gene in the Ovary of a Prenatally-Androgenized Rat Model of Polycystic Ovary Syndrome Following Androgen Exposure in the Prenatal Period. Iranian Journal of Endocrinology and Metabolism. 2021; 23 (2) :92-101
URL: http://ijem.sbmu.ac.ir/article-1-2888-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 23, Issue 2 (7-2021) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
Persian site map - English site map - Created in 0.04 seconds with 31 queries by YEKTAWEB 4414