Introduction: Lipocalin-2 as an adipokine expressed in adipose tissue has a direct relationship between systemic inflammation and insulin resistance in mice and humans. The aim of this study was to investigate the effect of progressive resistance training on serum lipocalin-2, non-HDL-c, and lipid and glycemic homeostasis in men. Materials and Methods: In this semi-experimental study, 20 healthy overweight and obese males (22.75±1.29 yr, 90.18±7.28 kg, 28.7±2.58 kg/m²) volunteered and were randomly assigned into the training (n=10) and the control (n=10) groups. Exercise protocol was progressive resistance training (8wk, 4d/wk, 60 min/d). Blood samples were taken before and after exercise to evaluate serum variables. Elisa method was used to measure plasma lipocalin-2 concentration. The T-test and Pearson correlation was employed for data analysis. Results: After 8 weeks’ progressive resistance training, no significant changes occured in serum levels of lipocalin-2 (p=0.59) and blood glycaemia (p=0.53), although levels of non-HDL-c (p=0.024), triglycerides (p=0.017), total cholesterol (p=0.032) and systolic blood pressure (p=0.002) were significantly decreased. There were no significant changes in other variables. A significant positive correlation was observed between changes of lipocalin-2 with insulin resistance index and a significant negative correlation with WHR in the post exercise compared to before. Conclusion: The results of this study, confirm the relationship between the lipocalin-2 insulin resistance and central obesity (WHR), with no significant impact on lipocalin-2, although it led to a significant reduction in some serum lipid profiles.