Introduction: Appetite influences energy homeostasis. Nesfatin-1 and Acylated ghrelin are orexigenic and anorexigenic peptides that play an important role in obesity and energy balance. The purpose of this study was to determine the influence of one cycle circuit resistance training on the Nesfatin-1 to Acylated ghrelin ratio in adolescent overweight. Materials and Methods: Twenty male students, age 18±0.92 years, weight 83.57±5.62 kg and BMI 27.48±1.5 kg/m2 (mean ±SE) were randomly allocated to the intervention and control groups. Subjects performead circuit-resistance training protocol with 60% 1RM, 3 sessions per week for 12 weeks. Levels of Plasma Nesfatin-1, Acylated ghrelin, Cortisol, Insulin and Growth hormone, were measured using the ELISA method. Results: Results revealed that after 12 weeks circuit resistance training, Nesfatin-1 (P=0.005), acylated ghrelin (P=0.02), the Nesfatin-1 to Acylated ghrelin ratio (P=0.008) and growth hormone (P=0.04) levels increased in the plasma significantly whereas cortisol (P=0.05) decreased significantly. A significant positive correlation (R=0.79) was found between Plasma Nesfatin-1 and GH whereas a significant negative correlation (R=-0.729) was seen between Plasma Nesfatin-1 and cortisol (P≤0.05). Conclusions: It seems that frequent circuit resistance training with moderate intensity increases levels of Nesfatin-1 and Acylated ghrelin, indicating that interaction between Nesfatin-1and acylated ghrelin may has a role in food intake and body weight modulation.