Introduction: Although Vitamin D deficiency is highly prevalent worldwide and has been suggested to be a contributory factor for cardiovascular disease in diabetes, the specific underlying mechanism is not known. It may be related to the effects of vitamin D deficiency on metabolic, inflammatory and hemostatic markers. Plasminogen activator inhibitor 1 is a known cardiovascular risk marker in diabetes. The aim of this study was to determine the association between 25 hydroxy vitamin D and PAI-1 in type 2 diabetic patients. Materials and Methods: One hundred-eighty patients with type 2 diabetes were selected, and the associations between PAI-1  and 25 hydroxy vitamin D level and other risk markers of cardiovascular disease including FBS, lipid profiles, HbA1C, creatinine and microalb­uminuria were examined. Results: There were no significant correlation between PAI-1 level and 25 hydroxy vitamin D level. PAI-1 was positively associated with fasting blood sugar and high density lipoprotein level, but no significant relation was found between PAI-1 level and other risk markers of cardiovascular disease. Conclusions: It seems that vitamin D deficiency is not related to PAI-1 level as a risk marker for cardiovascular disease in diabetes, but this observation remains to be confirmed by larger clinical studies.