Introduction: Heat shock proteins act as guardians of cells. The stimulation of these proteins due to stress conditions that activates the cell protective mechanisms is essential. Materials and Methods: In this study, in order to assess the effects of lead, regular exercise endurance and HSP72 protein supplement Curcumin on the liver tissue, 48 mice were classified randomly into control groups 1) Base and 2) Sham and four experimental groups included 3) Lead, 4) Lead + endurance training, 5) Lead + Curcumin, 6) Lead + endurance training + Curcumin. Groups of 3 to 6 received 20 mg of lead groups 5 and 6 in addition to lead received 30 mg/kg curcumin for 8 weeks and 3 days intraperitoneally. Furthermore, groups 4 and 6 performed progressive running training sessions, 15 to 22 m/min, for 25 to 64 min, five times a week HSP72 level in homogenized liver tissue was measured by ELISA method. Data were analyzed by one way ANOVA test, P≤0.05. Results: HSP72 and malondialdehyde levels in the lead group were significantly higher than the other groups, while superoxide dismutase and total anti-oxidative capacity were less than other groups. In contrast, HSP72 and malondialdehyde in the curcumin, endurance training and the combination groups were significantly lower than the lead groups. Conclusion: These findings suggest that healthy lifestyle, including aerobic exercise and anti oxidant supplements may have beneficial effects in preventing oxidative damage caused by lead.

Introduction: Glutamine plays a key role in cell protection following stress, by causing a simultaneous increase in hsp72 expression, which is dependent on glutamine metabolism and its relation with cortisol levels and gluconeogenesis. Materials and Methods: To assess the effect of glutamine supplement ingestion on hsp72, cortisol and glucose plasma changes after exercise, 28 soccer players were divided in four groups the control, supplement, supplement-exercise and the exercise groups. 0.5 g/kgBw supplement and placebo consumed 5ml/kgBw volume of water one hour prior to the exercise protocol, which included 3 stages of 20 minutes running (80% HRmax intensity) with 5 minute rest periods between each stage. Baseline, and pre, post and 90 minutes after exercise, blood sampling was done and cortisol, glucose and hsp72 levels were measured using RIA, Enzymatic, and Elisa tests respectively. Data was analyzed with MANOVA and Bonferoni post hoc tests, P≤0.05 values being considered significant. Results: Plasma cortisol and plasma glucose levels showed no significant changes in the groups, hsp72 while expression in the supplement and the supplement exercise groups was increased. Conclusion: Role of glutamine, independent of its relation with cortisol and glocostatic is a stimulator of hsp72 expression which is further increased by combining the supplement with exercise, suggesting that athletes may want to use glutamine prior to taking part in matches or intense exercises.