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Showing 1 results for Bax
B Amiri, Dr Mr Rahimi, Dr Sh Ahmadi, H Faraji,
Volume 26, Issue 1 (4-2024)
Abstract
Introduction: A high-fat diet (HFD) contributes to obesity and cardiac damage. This study investigated the protective effects of eight weeks of caffeine supplementation (CAF) on HFD-induced changes in the expression of BAX, BCL2, the BAX/BCL2 ratio, and caspase 3 in the heart tissue of male Wistar rats. Materials and Methods: Thirty-two male Wistar rats (8 weeks old, 181 ± 15 grams) were randomly assigned to one of four groups (n=8 per group): normal diet (ND), normal diet with caffeine (ND+CAF), high-fat diet (HFD), and high-fat diet with caffeine (HFD+CAF). Rats had free access to water and their assigned diet. The caffeine supplement was administered via gavage at 6 mg/kg/day. After eight weeks, heart tissue was collected, and the protein expression of apoptosis markers was measured using Western blotting. Data were analyzed using one-way ANOVA followed by Tukey’s post-hoc test (GraphPad Prism). Results: Compared to the ND and ND+CAF groups, the HFD and HFD+CAF groups showed significantly higher BAX expression (P≤0.001). BCL2 expression was significantly elevated in the ND+CAF and HFD+CAF groups compared to the ND and HFD groups (P≤0.001). The BAX/BCL2 ratio was lower in the ND+CAF and HFD+CAF groups compared to the ND and HFD groups (P ≤ 0.001). No significant differences were observed in caspase 3 expression (P≥0.05). Conclusion: These findings suggest that caffeine supplementation may mitigate HFD-induced alterations in the expression of specific apoptotic proteins in rat heart tissue
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