[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 13, Issue 3 (9-2011) ::
2011, 13(3): 288-293 Back to browse issues page
Association of polymorphisms G1193/C exon 8 and C2145/T exon 12 with Anti-TPO titer in Iranian population
Mehdi Hedayati Dr , Marzieh Salehi Jahromi, Laleh Hoghoughi, Marjan Zarif yeganeh, Maryam Daneshpour Dr, Fereidoun Azizi Dr
Obesity Research Center, Researcxh Institute for Endocrine Sciences , hedayati@endocrine.ac.ir
Abstract:   (28645 Views)


Introduction: Autoimmune thyroid diseases (AITD) are common and it is important to identify the genetic determinants. The aim of this study was to assess the relationship between two polymorphisms of Thyroid Peroxidase gene (TPO) and serum level of Anti-TPO titer in an Iranian population. Materials and methods: We selected 184 individuals from Tehran Lipid and Glucose Study, categorized as the Anti-TPO- (n=72) and Anti-TPO+ (n=112) groups. Inclusion criteria for cases was Anti-TPO and Anti-Tg>100U/L with a history of hypothyroidism. Anti-TPO levels in subjects were measured by the ELISA kit. Genomic DNA was extracted using Salting-out/Proteinase K method. Polymorphism detection of Exon 8 and 12 was done using the PCR-RFLP method. The PCR products were incubated with restriction enzymes SacII and BsrI, respectively. Results: The C allele frequency of C2145/T polymorphism Exon 12 (rs732608) was observed in 71.2% of patients and in 28.8% of normal individuals. This allele was significantly associated with increased levels of Anti-TPO [(T 140±330 pmol/L vs. C 436±380 pmol/L P<0.001), (OR: 9.2)]. The G1193/C was not associated with the level of serum Anti-TPO in this study. Conclusion: We demonstrated that the C allele polymorphism in C2145/T exon 12 is associated with high levels of serum Anti-TPO and that carriers of this allele are predisposed to disease 9.2 times more  than those who do not have A allele.

Keywords: Thyroid peroxidase antibody, Polymorphism, Thyroid Peroxidase gene, Autoimmune hypothyroidism
Full-Text [PDF 294 kb]   (2314 Downloads)    
Type of Study: Original | Subject: Genetic
Received: 2010/06/9 | Accepted: 2011/04/18
Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hedayati M, Salehi Jahromi M, Hoghoughi L, Zarif yeganeh M, Daneshpour M, Azizi F. Association of polymorphisms G1193/C exon 8 and C2145/T exon 12 with Anti-TPO titer in Iranian population. Iranian Journal of Endocrinology and Metabolism. 2011; 13 (3) :288-293
URL: http://ijem.sbmu.ac.ir/article-1-973-en.html

Volume 13, Issue 3 (9-2011) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
Persian site map - English site map - Created in 0.06 seconds with 33 queries by YEKTAWEB 4111