[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 10, Issue 4 (11-2008) ::
2008, 10(4): 331-339 Back to browse issues page
The Presence of Insulin Catalytic Antibodies in Plasma of Two Type 2 Diabetes Patients
M. Mohamdi , M Mirshahi , M Hedayati , M. Rahimzadeh Jahromi
, :mirshahi@modares.ac.ir
Abstract:   (29322 Views)

Abstract:

Introduction: Recent studies have reported the role of catalytic antibodies in the pathogenesis pattern of some diseases. Autoimmune reactions playing a role in some type 2 diabetic patients and the β- cell autoimmune markers were found to be present in some of these patients. The presence of such antibodies was assessed in the plasma of patients suffering from type 2diabetes. Material and Methods: Antibodies in 3 diabetic patients and 7 non-diabetic control subjects were purified using protein-G sepharose affinity column chromatography and S-300 gel filtration methods purity of the IgG antibodies was confirmed by SDS-PAGE and in western blot analysis and proteolytic activity of electrophoretically homogenous IgG antibodies was confirmed with zymogram analysis. The purified antibodies were incubated with insulin at 37°C for 6 days and the effect of antibodies on insulin degradation was assessed by Acetic Acid-Urea polyacrylamide gel electrophoresis. Results: Insulin degradation effect was observed only in purified antibodies in the 2 diabetic patients and it was not seen in the 7 control subjects and the remaining diabetic patients. Conclusion: Our data revealed, for the first time, insulin degradation by isolated IgG from 2 diabetic patients. This finding may not only explain the insulin resistance observed in some diabetic patients, but may most likely propose also a new mechanism for occurrence of the disease.

Keywords: Catalytic antibodies, Type 2 diabetes, Insulin, Autoimmune, Abzyme
Full-Text [PDF 476 kb]   (2435 Downloads)    
Type of Study: Original |
Received: 2008/11/26
Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohamdi M, Mirshahi M, Hedayati M, Rahimzadeh Jahromi M. The Presence of Insulin Catalytic Antibodies in Plasma of Two Type 2 Diabetes Patients. Iranian Journal of Endocrinology and Metabolism. 2008; 10 (4) :331-339
URL: http://ijem.sbmu.ac.ir/article-1-579-en.html


Volume 10, Issue 4 (11-2008) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3855