[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 10, Issue 1 (5-2008) ::
2008, 10(1): 59-66 Back to browse issues page
Inhibitory Effect of GABA on Glucose- Stimulated Insulin Secretion from Isolated Islets of Langerhans in Rat
F. Faraji , A. Ghasemi , M. Khosravi , F. Motamedi , S. Zahedi Asl
, zahedi@endocrine.ac.ir
Abstract:   (23217 Views)
Abstract Introduction: Gamma-amino butyric acid (GABA), an amino acid, present in some inhibitory neurons of the central nervous system, is also found in relatively high levels in the islets of Langerhans. Results of different studies concerning the effect of GABA on insulin secretion are controversial. The aim of this study was to determine the role of GABA and GABAB receptors on glucose-stimulated insulin secretion in isolated islets of rats. Materials and Methods: The collagenase digestion technique was used to isolate the islets from pancreata of 45 male Wistar rats (200-250g). Insulin secretion was assessed in eight islets in each cup exposed to different concentrations of glucose (8.3 and 16.7 mM) in the presence or absence of GABA(25, 50, 100 µM), baclofen (10, 20, 50 µM) (GABAB agonist) and saclofen (50,100 µM) (GABAB antagonist). Insulin concentration was measured by the ELISA method. Insulin release was reported as mean±SEM U/islet/50min and p values of <0.05 were considered significant. Results: GABA inhibited glucose (8.3 and 16.7 mM)-induced insulin secretion in isolated islets (P<0.05). Different concentrations of baclofen had no significant effect on glucose-induced insulin secretion however glucose (16.7mM) stimulated insulin secretion in the presence of 100 µM saclofen (91±8.8 µU/islet/60min) was significantly (p<0.05) higher compared to insulin secretion stimulated by 16.7mM glucose alone (67.7±2.58 µU/islet/60min). Conclusion: These findings indicate that GABA has an inhibitory effect on glucose-induced insulin secretion and therefore it may play a regulatory role in insulin secretion. This effect needs to be taken in to account in the pathophysiology of diabetes.
Keywords: GABA, Baclofen, Saclofen, Insulin, Glucose, Langerhans islets, Rats
Full-Text [PDF 326 kb]   (2780 Downloads)    
Type of Study: Original | Subject: Endocrinology
Received: 2008/06/23
Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Faraji F, Ghasemi A, Khosravi M, Motamedi F, Zahedi Asl S. Inhibitory Effect of GABA on Glucose- Stimulated Insulin Secretion from Isolated Islets of Langerhans in Rat. Iranian Journal of Endocrinology and Metabolism. 2008; 10 (1) :59-66
URL: http://ijem.sbmu.ac.ir/article-1-494-en.html


Volume 10, Issue 1 (5-2008) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
Persian site map - English site map - Created in 0.05 seconds with 33 queries by YEKTAWEB 3855