Introduction: It seems that, relatively studies have examined the effects of strength training on irisin and myostatin hormones and to date, the association between irisin and myostatin with blood lipids in response to strength training has been assessed. Therefore, the purpose of this study was to investigate the effect of strength training on serum level of irisin and myostatin hormones, and their association with lipid profile in untrained women. Materials and Methods: In a semi experimental study 16 active untrained women were randomly assigned into two, the training (n=10; body mass index: 23.45±2.83 kg/m2) and the control (n=6; age; body mass index: 23.28±2.62 kg/m2) groups. The strength training program consisted of 8 weeks, three sessions per week, each session 65 minutes. Serum levels of irisin, myostatin and lipid profile concentrations were measured before and 24 hours the after last training session. Data analyses were performed using SPSS version 22 and significance was assigned at P<0.05. Results: Results showed significant decrease in levels of cholesterol and myostatin in the training group (P<0.05) with a strong correlation between irisin and myostatin levels after 8 weeks of training (P<0.05). However no correlation were seen between irisin and myostatin with lipid profile (p>0.05). Conclusion: In conclusion the results of the study demonstrated that strength training can have favorable effects on myostatin and cholesterol serum levels in untrained women and that myostatin level is strongly correlated with irisin.