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:: Volume 14, Issue 5 (1-2013) ::
2013, 14(5): 498-503 Back to browse issues page
The in Vitro Effect of sICAM-1 on Oxidation of LDL and HDL and Activity of Paraoxonase-1 Enzyme
Nayeri Hashem Nayeri , Gholamali Naderi , Adel Abedpour , Maryam Boshtam , Narges Jafari Dinani
Abstract:   (6051 Views)

Introduction: The intercellular adhesion molecule-1 (ICAM-1) plays a pivotal role in inflammation and linkage between monocytes and endothelium. LDL-induced ICAM-1 expression may include the possibility of an atherogenic stimulation due to a binding between lipoprotein and ICAM-1. To evaluate of this hypothesis, the present study investigated the effect of sICAM-1 on susceptibility of LDL and HDL to oxidation and Paraoxonase-1 (PON-1) activity. Materials and Methods: LDL and HDL were purified from serum via ultracentrifugation and 25 and 100 µg-protein/ml of LDL and HDL, in the presence of sICAM-1 (0, 2, 5 ng/ml), were exposed to oxidative stress (5 µM copper) and assayed through monitoring of formation of conjugated dienes using a spectrophotometer. Electrophoresis was performed to determine the mobility of incubated-lipoproteins with sICAM-1. The activity of PON-1 was assayed in the presence of of sICAM-1. Results: In presence of different concentrations of sICAM-1, Vmax increased significantly (P<0.05), while electrophoretic mobility of LDL decreased (↓ 25%). PON-1 activity increased at low levels of sICAM-1 but decreased at higher levels (P<0.05 P<0.005). Conclusion: The results indicate that sICAM-1 in dosages over than 2  ng/ml affects some kinetical parameters of LDL oxidation and PON-1 activity that can be interpreted as a positive stimulus in atherogenesis. Overall the results may support the hypothesis of a lipoprotein-mediated role for ICAM-1 in atherogenesis. of

Keywords: ICAM-1, LDL, HDL, Ox-LDL, Paraoxonase-1
Full-Text [PDF 295 kb]   (1580 Downloads)    
Type of Study: Original | Subject: Cardiology
Received: 2012/03/11 | Accepted: 2012/07/15
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Nayeri N H, Naderi G, Abedpour A, Boshtam M, Jafari Dinani N. The in Vitro Effect of sICAM-1 on Oxidation of LDL and HDL and Activity of Paraoxonase-1 Enzyme. Iranian Journal of Endocrinology and Metabolism. 2013; 14 (5) :498-503
URL: http://ijem.sbmu.ac.ir/article-1-1353-en.html


Volume 14, Issue 5 (1-2013) Back to browse issues page
مجله ی غدد درون‌ریز و متابولیسم ایران، دو ماهنامه  پژوهشی مرکز تحقیقات غدد درون‌ریز و متابولیسم، Iranian Journal of Endocrinology and Metabolism
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