fa تاثیر چاقی و کاهش وزن ناشی از تمرین‌های استقامتی بر بیان UCP3 mRNA در موش‌های نژاد C57BL/6 ورزش Exercise پژوهشی Original <p style="LINE-HEIGHT: 16pt TEXT-INDENT: 3.7pt MARGIN: 0cm 36pt 0pt 1cm mso-line-height-rule: exactly" dir="rtl" class="Abstract"><strong><u><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA">مقدمه</span></u><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA">: هدف پژوهش حاضر، بررسی اثر چاقی و کاهش وزن ناشی از تمرین‌های استقامتی بر بیان ژن </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">UCP3 mRNA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> در موش­های چاق نژاد</span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">C57BL/6 </span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span><font face="Lotus"><span style="mso-spacerun: yes"><font size="3"> </font></span>بود. </font><u>مواد و روش‌ها</u>: 24 سر موش نژاد </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">C57BL/6</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt"><span dir="rtl"></span> <span lang="FA"><font size="3" face="Lotus">به مدت 12 هفته از غذای پرچرب و 6 سر دیگر (گروه </font></span></span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">NB</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) نیز از غذای معمولی تغذیه کردند. 6 سر موش چاق (گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">OB</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) پس از دوره چاق شدن کشته شدند.12 سر پس از دوره چاق شدن در دو گروه جداگانه تمرین با شدت بالا (گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">HI</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) (معادل 75% </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">VO2max</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) و تمرین با شدت پائین (گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">LI</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) (معادل 50% </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">VO2max</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) به مدت 12 هفته و هر هفته 5 جلسه تمرین کردند. گروه کنترل (گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">OC</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>) نیز تا پایان دوره بدون هیچ فعالیتی در قفس نگه‌داری شدند. وزن موش­ها به صورت هفتگی اندازه‏گیری و ثبت شد. بیان ژن</span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">UCP3</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> در سطح </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">mRNA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> در عضله‌ی دوقلوی موش­ها با استفاده از روش </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">Real Time PCR</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> و </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">FFA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> پلاسما به روش الایزا اندازه‏گیری شدند. <u>یافته­ها</u>: کاهش وزن گروه­های تمرینی از هفته‌ی 8 کاهش معنی‌داری داشت (05/0></span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">P</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>). میانگین میزان بیان نسبی </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">UCP3 mRNA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> در گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">HI</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> و </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">LI</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> به طور معنی­داری کمتر از گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">OC</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> بود (25/0±5/0 و96/0±04/1 واحد در مقابل 57/0±58/2 واحد، 05/0></span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">P</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>). میانگین </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">FFA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> در گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">OB</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> و </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">HI</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> به طور معنی­داری کمتر از گروه </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">LI</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> بود (05/0></span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">P</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span>). <u>نتیجه‌گیری</u>: زمانی که انرژی دریافتی افزایش یافته و در پی آن وزن بدن افزایش می­یابد، </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">UCP3</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> نیز افزایش می‌یابد تا گرمازایی و هم‌چنین اکسیداسیون اسیدهای چرب را افزایش دهد. اما در مقابل، با کاهش وزن ناشی از فعالیت ورزشی، مقادیر </span><font face="Arial"><span style="mso-font-kerning: 16.0pt" dir="ltr">UCP3</span><span dir="rtl"></span></font><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-ansi-font-size: 9.0pt mso-font-kerning: 16.0pt" lang="FA"><span dir="rtl"></span> نیز کاهش می‌یابد تا با این روند مقابله کند.<p></p></span></strong></p> <p style="TEXT-ALIGN: justify MARGIN: 0cm 1cm 0pt unicode-bidi: embed DIRECTION: ltr" class="Abstract"><strong><font face="Arial">Abstract</font></strong></p><p style="TEXT-ALIGN: justify MARGIN: 0cm 1cm 0pt unicode-bidi: embed DIRECTION: ltr" class="Abstract"><strong><font face="Arial"><u>Introduction</u>: The purpose of this study was evaluating the effect of obesity and weight reduction induced by endurance training on UCP3 mRNA expression in C57BL/6 mice. <u>Materials and Methods</u>: Twenty-four C57BL/6 mice consumed a high-fat diet, while 6 (NB Group) were fed a normal chow for 12 weeks. After the fattening period, 6 obese mice (OB Group) were sacrificed. Twelve mice trained 5 sessions per week for 12 weeks on a rodent treadmill with high (HI Group) (~75% VO2max) and/or low intensity (LI Group) (~50% VO2max) during a training period. The control group (OC Group) was kept inactive in the cages till the end of the period. Body weight was measured and recorded weekly. UCP3 mRNA expression was measured in gastrocnemius muscle using Real Time-PCR method and plasma FFA concentrations were measured using the ELISA method. <u>Results</u>: In the training groups, weight reduction was significant in the 8th week (p<0.05). Average relative UCP3 mRNA expression was significantly lower in the HI and LI groups, than in the OC group (0.5±0.25 and 1.04±0.96, respectively, vs. 2.58±0.57 arbitrary unit p<0.05). Plasma FFA conce­ntrations were significantly lower in the HI and <place w:st="on">OB</place> groups compared to the LI group (p<0.05). <u>Conclusion</u>: When energy intake and consequently body weight increases, UCP3 increases to extend the thermogenesis and fatty acid oxidation’s process. In contrast, UCP3 downregulates with exercise-induced weight loss in order to counteract this process.</font></strong></p><p></p> چاقی, کاهش وزن, تمرین‌های استقامتی, UCP3 mRNA Obesity, Weight loss, Endurance training, UCP3 mRNA 311 321 http://ijem.sbmu.ac.ir/browse.php?a_code=A-10-1500-1&slc_lang=fa&sid=1 Hamid Mohebbi حمید محبی mohebbi_h@yahoo.com 4000319475328460022425 4000319475328460022425 Yes University of Guilan دانشگاه گیلان Hadi Rohani هادی روحانی h_rohani7@yahoo.com 4000319475328460022426 4000319475328460022426 No University of Guilan دانشگاه گیلان Sadegh Hassan-nia صادق حسن نیا hasannia1967@gmail.com 4000319475328460022427 4000319475328460022427 No University of Tarbiat Modares دانشگاه تربیت مدرس Nazanin Pirooznia نازنین پیروزنیا nazanin_pirooznia@yahoo.com 4000319475328460022428 4000319475328460022428 No University of Guilan دانشگاه گیلان