|
Protective Effect of Vitamin E on Diabetes Induced Apoptosis and Oxidative Stress in Rat Heart Tissue
|
A. Shiroo   , S. Salami , M. Khadem Ansari , F. Ghaderi Pakdel , K. Khadem Vatani , R. Saadatian , M. Karimipour |
| , ashirpoor@yahoo.com |
|
|
Abstract: (30669 Views) |
| Abstract Introduction: The purpose of this study was to determine and to assess the protective effect of vitamin E on cardiomyocyte apoptosis and oxidative stress status in the heart under hyperglycemic conditions, in vivo. Materias and Methods: Wistar male rats (n=16) at 6 months of age were made hyperglycemic by STZ. Same age, normal wistar rats (n=8) were used for comparison (controls). Diabetic rats were divided into two groups, the nontreated and those treated with vitamin E (300mg/kg/daily). Results: Diabetic rats exhibited severe apoptosis in cardiomyocytes. Also significant increases in lipid peroxidation as measured by 8- isoprostan, protein oxidation as measured by protein carbonyl content and superoxide dismutase were observed after 6 weeks. Catalase activity was shown to increase in controls compared to nontreated rats. A distinct elevation in the HbA1C, QT interval and a decline in the activity of catalase were also observed. Vitamin E treated rats shown significant decline in apoptosis, lipid peroxidation, protein carbonyl and QT interval compared to nontreated rats. Conclusion: Vitamin E decreased the incidence of apoptosis in cardiomyocytes, lipid peroxidation and improve antioxidant enzyme in the diabetic hearts of rats. Further research to confirm the findings is recommended. |
|
| Keywords: Diabetes, Vitamin E, Wistar rat, Cardiomyocyte, Apoptosis, Oxidative stress |
|
|
Full-Text [PDF 399 kb]
(5440 Downloads)
|
Type of Study: Original |
Subject:
Endocrinology
Received: 2008/06/23 | Published: 2008/05/15
|
|
|
|
|
|
