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Protective Effect of Vitamin E on Diabetes Induced Apoptosis and Oxidative Stress in Rat Heart Tissue
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| A. Shiroo, S. Salami, M. Khadem Ansari, F. Ghaderi Pakdel, K. Khadem Vatani, R. Saadatian, M. Karimipour |
| , ashirpoor@yahoo.com |
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Abstract: (29128 Views) |
| Abstract Introduction: The purpose of this study was to determine and to assess the protective effect of vitamin E on cardiomyocyte apoptosis and oxidative stress status in the heart under hyperglycemic conditions, in vivo. Materias and Methods: Wistar male rats (n=16) at 6 months of age were made hyperglycemic by STZ. Same age, normal wistar rats (n=8) were used for comparison (controls). Diabetic rats were divided into two groups, the nontreated and those treated with vitamin E (300mg/kg/daily). Results: Diabetic rats exhibited severe apoptosis in cardiomyocytes. Also significant increases in lipid peroxidation as measured by 8- isoprostan, protein oxidation as measured by protein carbonyl content and superoxide dismutase were observed after 6 weeks. Catalase activity was shown to increase in controls compared to nontreated rats. A distinct elevation in the HbA1C, QT interval and a decline in the activity of catalase were also observed. Vitamin E treated rats shown significant decline in apoptosis, lipid peroxidation, protein carbonyl and QT interval compared to nontreated rats. Conclusion: Vitamin E decreased the incidence of apoptosis in cardiomyocytes, lipid peroxidation and improve antioxidant enzyme in the diabetic hearts of rats. Further research to confirm the findings is recommended. |
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| Keywords: Diabetes, Vitamin E, Wistar rat, Cardiomyocyte, Apoptosis, Oxidative stress |
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Full-Text [PDF 399 kb]
(4993 Downloads)
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Type of Study: Original |
Subject:
Endocrinology
Received: 2008/06/23 | Published: 2008/05/15
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