Introduction: The aim of this study was to investigate the effect of moderate and high volume aerobic training on the expression of Uncoupling Protein 1(UCP-1) gene in subcutaneous WAT (sub-WAT). Materials and Methods: Twenty-four rats were assigned randomly into three groups: 1) control (n=8) 2) moderate-volume aerobic training (n=8) and 3) high-volume aerobic training (n=8). Subjects of training groups underwent continuous aerobic training on the treadmill for 8 weeks, 5 sessions per week at two different volumes of training. The Real Time–PCR method was used to measure the expression ratio of UCP-1 gene. Results: Data showed that although the expression ratio of UCP1 gene in the moderate volume aerobic training group was significantly higher than control group (P=0.014), its expression ratio in the high volume aerobic training group did not differ significantly from controls (P=0.36); neither was the gene expression ratio different between moderate and high volume aerobic training groups (P=0.59). Conclusion: Results indicate that moderate volume aerobic training, had an obvious effect in inducing UCP1 gene expression in subcutaneous adipose tissue, while the high volume aerobic training did not. Thus, increasing the volume of aerobic training to high levels may not be a key factor in promoting the non-shivering theremogenesis of sub-WAT.