Introduction: Selectin molecules are responsible for leukocyte adhesion to vascular endothelium. We evaluated the effects of eight weeks high-intensity interval training (HIIT) on E-selectin and P- selectin in obese women. Materials and Methods: In this quasi-experimental study, 16 obese women participants (age: 28.06±3.62 years, BMI: 31.49±5.15 kg/m2) using the  convenience sampling method, were randomly categorized into two groups (n=8 each), the experimental and the controls. In the trained group, the volunteers participated in 8 weeks HIIT, 3 days/week the HIIT program training included: 45-60 min duration with 90% HRR per session. The Rockport test was used to estimate maximal oxygen consumption. Blood samples were taken from the participants at the beginning end of 8 weeks in order to measure factors such as (E-selectin and P- selectin). Data were analyzed using paired and independent sample t-test for comparison of means within and between groups respectively, with level of significance set at p≤0.05. Results: Weight and BMI levels reduced, although changes were not significant. WHR reduced significantly at the end of the 8 week period (p=0.007) and Vo2max increased significantly. (p=0.00). In the aerobic exercise group, E-selectin decreased from 89.76 to 84.27 ng per ml and P-selectin from 7.88 to 7.47 ng per ml, again the difference not statistically significant (p>0.05). Conclusion: Considering reduction in weight and selectin molecules and increased Vo2max, following HIIT training, this can be used in as therapy for management of cardiovascular patients.