Introduction: In present study, we examined the effects of endurance training alone and endurance training combined with taking garlic supplement on serum homocysteine levels, complex plasminogen activator (TPA), and body composition parameters such as weight, BMI and body fat percentage in sedentary women. Materials and Methods: Twenty-seven women who used to Alzahra sport clubs of 3 and 4 municipality of Tehran participated in this study (age, 38.53±7.59 years; weight, 77.61±5.26 kg). The subjects were randomly divided into three groups, endurance training, endurance training combined with garlic supplement and the control groups. The training protocol included: Running on a treadmill for 60 minutes at 60 to 75% of maximum heart rate for 10 weeks 5 times a week. Subjects of the training combined with garlic supplement group, received 2 tablets daily (in the form of capsules, 500 mg, nature made). At the beginning and 48 hours after training, the subjects were assessed for homocysteine and TPA. Data were analyzed using paired t-test and one way ANOVA (P<0.05). Results: Findings showed that in the endurance training and the endurance training combined with garlic supplement groups, serum homocysteine levels were significantly decreased (p<0.05). Although, other variables were reduced, but these changes were not statistically significant. Conclusion: According to this study, 10 weeks of endurance exercise with reduction of homocysteine levels, could lead to reduction in the risk of cardiovascular disease in sedentary obese women.