Introduction: In this study, the effect of continuous aerobic training on serum indices of liver tissue damage enzymes (aspartate aminotransferase (AST), alanine amino transferase (ALT), and alkaline phosphatase (ALP)) in rats with myocardial infarction (MI) were evaluated. Material and Methods: For this purpose, four weeks after MI, 30 male Wistar rats were randomized to the following groups: 1: Sham (n=10); 2: MI-sedentary (Sed-MI), n=10 and 3: MI-exercise (Ex-MI), n=10 the rats were subjected to aerobic training in the form of treadmill running for 10 weeks (5 days per week) at 17 m/min for 50 min/day. At the end of training program, serum AST, ALT, and ALP were measured by ELISA kits. Results were analyzed by one-way analysis of variance (ANOVA) and P<0.05 was considered as significant level. Results: AST activity, in the MI-sedentary and MI-exercise groups was significantly higher at the end of training (p=0.01), there was however no significant difference between MI-sedentary and MI-exercise (p=0.93). Alanine amino transferase was also significantly increased after MI (p=0.02), there was howere no significant difference between MI-exercise and the MI-sedentary groups (p=0.55). ALP activity was comparable between groups (p=0.9). Conclusion: Ten weeks continues aerobic exercise training with moderate intensity had no effect on liver function in rats with MI. To better evaluate the effect of exercise training on liver pathological indices immunohistochemical analyses are needed.