:: Volume 4, Issue 2 (6-2002) ::
2002, 4(2): 117-120 Back to browse issues page
A Study on the fragility of red blood cells in hypothyroid rats
S Zahedi , F Nabavizadeh
, zahedi@erc-iran.com
Abstract:   (25915 Views)
Introduction: Hypothyroidism causes many hematologic abnormalities including anemia. Anemia can be due to iron deficiency, impaired hemoglobin synthesis, poor diet, structural abnormalities of the red blood cells (RBC) and reduced half-life of RBC. In this study RBC fragility in hypothyroid rats were compared to that of control animals. Materials and method: Hypothyroidism was induced by methimazole (500 mg/L of drinking water for 20 days) in the rats (body weight, 246±5gr). Blood samples were collected through carotid vein on EDTA for osmotic fragility test. RBC fragility of RBC was tested by a routine method in which the cells were placed in different concentrations of NaCl (0.09 gr/L) and hemolysis was determined by colorimetric method. The hemolysis precentage of the samples in each solution was calculated on the basis of the 100% hemolysis in distilled water. Results: RBCs from hypothyroid animals had significantly (p<0.05) higher hemolysis in NaCl concentrations of 2.5, 3, 4, 4.5, 5, 5.5, 6 and 7 gr/L as compared to control animals. Conclusion: Results of this study prove that anemia in hypothyroid rats could also be due to increased fragility of RBC.
Keywords: Anemia, Hypothyroidism, Rats, Fragility, Red blood Cells.
Full-Text [PDF 245 kb]   (5871 Downloads)    
Type of Study: Original | Subject: Endocrinology
Received: 2006/11/21 | Published: 2002/06/15


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Volume 4, Issue 2 (6-2002) Back to browse issues page