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Effect of six weeks endurance training on tumor tissue IL-10 cytokine levels in breast cancer bearing mice
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Yaghoub Shiri   , Hamid Agha-Alinejad , Reza Gharakhanlou , Sadegh Amani Shalamzari , Mohammad Amin Saei |
| Tarbiat Modares University , halinejad@modares.ac.ir |
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Abstract: (8449 Views) |
| Background: The aim of this study was evaluate the effects of endurance training on tissue IL-10 cytokine level in breast cancer bearing mice. Methods: The twenty female Balb/c mice (6-8 week-old, 14±2 gr weights) were divided to two control and exercise groups. After orientation in the environment, estrogen-receptor dependent breast cancer cells (MC4-L2) were injected to them and exercise group performed endurance exercise for 6 weeks. After appearance of the tumor, the length and width of the tumor were measured by a digital caliper once a week. Finally, the mice were sacrificed tumor tissue was removed and immediately frozen and kept in -70°C. Tumor sample was homogenized and levels of IL-10 were measured and quantified using ELISA method. Results: There was significant increase on level of IL-10 in exercise group than control group (P≤0.05). Tumor volume reduced in exercise group than control group. The results showed that the amount of IL-10 significantly increased in the exercise group compared to control group Also reduction in tumor volume observed in the exercise group compared to control group. Conclusion: Increased levels of IL-10 was in parallel to reduction in tumor volume, which shows that exercise lead to reduction tumor growth through increased levels of this cytokine. Angiogenesis is a prerequisite for continued growth and spread of tumors, therefore, increased levels of IL-10 that is an angiogenesis cytokine in the exercise group compared to the control group shows exercise can inhibit from tumor growth by modulating cytokine profile within the tumor microenvironment. |
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| Keywords: Breast cancer, endurance training, interleukin-10, tumor volume |
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Full-Text [PDF 280 kb]
(3465 Downloads)
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Type of Study: Original |
Subject:
Exercise
Received: 2013/08/23 | Accepted: 2014/05/14 | Published: 2014/09/9
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